A Smooth-Type, Phage-Resistant Klebsiella pneumoniae Mutant Strain Reveals that OmpC Is Indispensable for Infection by Phage GH-K3 | Zendy

Ruopeng Cai | Zendy; Mei Wu | Zendy; Hao Zhang | Zendy; Yufeng Zhang | Zendy; Mengjun Cheng | Zendy; Zhimin Guo | Zendy; Yalu Ji | Zendy; Hengyu Xi | Zendy; Xinwu Wang | Zendy; Yibing Xue | Zendy; Changjiang Sun | Zendy; Xin Feng | Zendy; Liancheng Lei | Zendy; Yigang Tong | Zendy; Xiaoyun Liu | Zendy; Wenyu Han | Zendy; Jingmin Gu | Zendy

Open Access

A Smooth-Type, Phage-Resistant Klebsiella pneumoniae Mutant Strain Reveals that OmpC Is Indispensable for Infection by Phage GH-K3

Author(s) -

Ruopeng Cai,

Mei Wu,

Hao Zhang,

Yufeng Zhang,

Mengjun Cheng,

Zhimin Guo,

Yalu Ji,

Hengyu Xi,

Xinwu Wang,

Yibing Xue,

Changjiang Sun,

Xin Feng,

Liancheng Lei,

Yigang Tong,

Xiaoyun Liu,

Wenyu Han,

Jingmin Gu

Publication year - 2018

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01585-18

Subject(s) - biology , klebsiella pneumoniae , bacteriophage , complementation , microbiology and biotechnology , mutant , mutation , proteinase k , klebsiella , gene , escherichia coli , dna , genetics

With increased incidence of multidrug-resistant (MDR) bacterial strains, phages have regained attention as promising potential antibacterial agents. However, the rapid emergence of resistant variants during phage treatment has limited the therapeutic applications of phage. According to ourtrans -complementation,ompC mutation, and phage adsorption efficiency assays, we identified OmpC as the key receptor-binding protein (RBP) for phage GH-K3, which is essential for effective infection. This study revealed that the phage secondary receptor ofK. pneumoniae , OmpC, is the essential RBP not only for phage infecting Gram-negative bacteria, such asEscherichia coli andSalmonella , but also forK. pneumoniae .

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research