Field Detection of Schistosoma japonicum Cercariae in Environmental Water Samples by Quantitative PCR
Author(s) -
Caitlin M. Worrell,
Ning Xiao,
Jorge E. Vidal,
Lin Chen,
Bo Zhong,
Justin V. Remais
Publication year - 2011
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01561-10
Subject(s) - schistosoma japonicum , biology , bioassay , real time polymerase chain reaction , sampling (signal processing) , quantitative analysis (chemistry) , schistosomiasis , ecology , chromatography , helminths , chemistry , zoology , biochemistry , gene , filter (signal processing) , computer science , computer vision
A species-specific quantitative PCR (qPCR) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection of S. japonicum in surface waters. The novel methods were capable of capturing cercariae and, with subsequent analysis through qPCR, detecting the presence of a minimum of 1 cercaria.
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