Open AccessCofactor Trapping, a New Method To Produce Flavin Mononucleotide
Author(s) -
Ulrich Krauß,
Vera Svensson,
Astrid Wirtz,
Esther Knieps-Grünhagen,
KarlErich Jaeger
Publication year - 2011
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01541-10
Subject(s) - flavin mononucleotide , flavoprotein , cofactor , flavin group , escherichia coli , riboflavin , flavin adenine dinucleotide , chemistry , nicotinamide mononucleotide , biochemistry , trapping , enzyme , biology , nad+ kinase , gene , nicotinamide adenine dinucleotide , ecology
We have purified flavin mononucleotide (FMN) from a flavoprotein-overexpressing Escherichia coli strain by cofactor trapping. This approach uses an overexpressed flavoprotein to trap FMN, which is thus removed from the cascade regulating FMN production in E. coli. This, in turn, allows the isolation of highly pure FMN.