Cofactor Trapping, a New Method To Produce Flavin Mononucleotide | Zendy

Ulrich Krauß | Zendy; Vera Svensson | Zendy; Astrid Wirtz | Zendy; Esther KniepsGrünhagen | Zendy; KarlErich Jaeger | Zendy

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Cofactor Trapping, a New Method To Produce Flavin Mononucleotide

Author(s) -

Ulrich Krauß,

Vera Svensson,

Astrid Wirtz,

Esther KniepsGrünhagen,

KarlErich Jaeger

Publication year - 2010

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01541-10

Subject(s) - flavin mononucleotide , flavoprotein , cofactor , flavin group , escherichia coli , riboflavin , flavin adenine dinucleotide , chemistry , nicotinamide mononucleotide , biochemistry , trapping , enzyme , biology , nad+ kinase , gene , nicotinamide adenine dinucleotide , ecology

We have purified flavin mononucleotide (FMN) from a flavoprotein-overexpressing Escherichia coli strain by cofactor trapping. This approach uses an overexpressed flavoprotein to trap FMN, which is thus removed from the cascade regulating FMN production in E. coli. This, in turn, allows the isolation of highly pure FMN.

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