Effect of Freezing on PCR Amplification of 16S rRNA Genes from Microbes Associated with Black Band Disease of Corals | Zendy

Raju Sekar | Zendy; Longin T. Kaczmarsky | Zendy; Laurie L. Richardson | Zendy

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Effect of Freezing on PCR Amplification of 16S rRNA Genes from Microbes Associated with Black Band Disease of Corals

Author(s) -

Raju Sekar,

Longin T. Kaczmarsky,

Laurie L. Richardson

Publication year - 2009

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01500-08

Subject(s) - 16s ribosomal rna , biology , ribosomal rna , microbiology and biotechnology , gene , genetics

Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.

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