TaqMan-Based Real-Time PCR Method for Detection of Yersinia pseudotuberculosis in Food | Zendy

Susanne Thisted Lambertz | Zendy; C. Nilsson | Zendy; Saija Hallanvuo | Zendy

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TaqMan-Based Real-Time PCR Method for Detection of Yersinia pseudotuberculosis in Food

Author(s) -

Susanne Thisted Lambertz,

C. Nilsson,

Saija Hallanvuo

Publication year - 2008

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01459-08

Subject(s) - yersinia pseudotuberculosis , taqman , primer (cosmetics) , biology , yersinia , yersinia enterocolitica , gene , microbiology and biotechnology , polymerase chain reaction , computational biology , genetics , bacteria , chemistry , organic chemistry , virulence

A sensitive and specific assay for detection of food-borne pathogenicYersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located geneail . The complete method, including an internal amplification control, was evaluated for several different food items.

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