Open AccessTaqMan-Based Real-Time PCR Method for Detection of Yersinia pseudotuberculosis in Food
Author(s) -
S. Thisted Lambertz,
C. Nilsson,
Saija Hallanvuo
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01459-08
Subject(s) - yersinia pseudotuberculosis , taqman , primer (cosmetics) , biology , yersinia , yersinia enterocolitica , gene , microbiology and biotechnology , polymerase chain reaction , computational biology , genetics , bacteria , chemistry , organic chemistry , virulence
A sensitive and specific assay for detection of food-borne pathogenicYersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located geneail . The complete method, including an internal amplification control, was evaluated for several different food items.