The Functional Quality of Soluble Recombinant Polypeptides Produced in Escherichia coli Is Defined by a Wide Conformational Spectrum | Zendy

Mónica MartínezAlonso | Zendy; Núria GonzálezMontalbán | Zendy; Elena GarcíaFruitós | Zendy; Antonio Villaverde | Zendy

Open Access

The Functional Quality of Soluble Recombinant Polypeptides Produced in Escherichia coli Is Defined by a Wide Conformational Spectrum

Author(s) -

Mónica MartínezAlonso,

Núria GonzálezMontalbán,

Elena GarcíaFruitós,

Antonio Villaverde

Publication year - 2008

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01446-08

Subject(s) - escherichia coli , recombinant dna , biochemistry , fluorescence , biology , chemistry , biophysics , gene , physics , quantum mechanics

We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure.

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