Open AccessThe Functional Quality of Soluble Recombinant Polypeptides Produced in Escherichia coli Is Defined by a Wide Conformational Spectrum
Author(s) -
Maira Martínez-Alonso,
Nuria Gonzàlez-Montalbán,
Elena GarcíaFruitós,
Antonio Villaverde
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01446-08
Subject(s) - escherichia coli , recombinant dna , biochemistry , fluorescence , biology , chemistry , biophysics , gene , physics , quantum mechanics
We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure.