Participation of Valine 171 in α-Helix 5 of Bacillus thuringiensis Cry1Ab δ-Endotoxin in Translocation of Toxin into Lymantria dispar Midgut Membranes | Zendy

Óscar Álzate | Zendy; Cristina Osorio | Zendy; Álvaro M. Flórez | Zendy; Donald H. Dean | Zendy

Open Access

Participation of Valine 171 in α-Helix 5 of Bacillus thuringiensis Cry1Ab δ-Endotoxin in Translocation of Toxin into Lymantria dispar Midgut Membranes

Author(s) -

Óscar Álzate,

Cristina Osorio,

Álvaro M. Flórez,

Donald H. Dean

Publication year - 2010

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01428-10

Subject(s) - lymantria dispar , bacillus thuringiensis , midgut , manduca sexta , biology , toxin , microbiology and biotechnology , dispar , bacillales , insect , biochemistry , botany , bacteria , larva , genetics , entamoeba histolytica , bacillus subtilis

The Cry1Ab δ-endotoxin V171C mutant protein exhibits a 25-fold increase in toxicity against Lymantria dispar, which correlates with a faster rate of partitioning into the midgut membrane and slightly decreased protein stability. This is an insect-specific mechanism; similar results were not observed in Manduca sexta, another Cry1Ab δ-endotoxin-susceptible insect.

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