Boosting Secretion of Extracellular Protein by Escherichia coli via Cell Wall Perturbation

Cell surface structure stabilization is important for extracellular secretion of proteins inEscherichia coli . As the main constituent of the cell wall, peptidoglycan contributes to cell structure robustness and stability. Here, we perturbed the peptidoglycan network by deletingdacA anddacB genes encodingd ,d -carboxypeptidase enzymes to improve extracellular protein secretion. This new strategy could enhance the capacity ofE. coli as a microbial cell factory for extracellular secretion of proteins and chemicals.