CRISPR/Cas9-Assisted Seamless Genome Editing in Lactobacillus plantarum and Its Application in N -Acetylglucosamine Production | Zendy

Ding Zhou | Zendy; Zhennan Jiang | Zendy; Qingxiao Pang | Zendy; Yuan Zhu | Zendy; Qian Wang | Zendy; Qingsheng Qi | Zendy

Open Access

CRISPR/Cas9-Assisted Seamless Genome Editing in Lactobacillus plantarum and Its Application in N -Acetylglucosamine Production

Author(s) -

Ding Zhou,

Zhennan Jiang,

Qingxiao Pang,

Yuan Zhu,

Qian Wang,

Qingsheng Qi

Publication year - 2019

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01367-19

Subject(s) - recombineering , genome editing , crispr , lactobacillus plantarum , plasmid , cas9 , biology , dna , gene , computational biology , bacteria , genetics , lactic acid

CRISPR/Cas9-assisted recombineering is restricted in lactic acid bacteria because of the lack of available antibiotics and vectors. In this study, a seamless genome editing method was carried out inLactobacillus plantarum using CRISPR/Cas9-assisted double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) recombineering, and recombination efficiency was effectively improved by endogenous adenine-specific methyltransferase overexpression.L. plantarum WCFS1 produced 797.3 mg/literN -acetylglucosamine (GlcNAc) through reinforcement of the GlcNAc pathway, without introducing exogenous genes or plasmids. This seamless editing strategy, combined with the potential exogenous GlcNAc-producing pathway, makes this strain an attractive candidate for industrial use in the future.

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