Genetic Diversity of Native Bradyrhizobia Isolated from Soybeans ( Glycine max L.) in Different Agricultural-Ecological-Climatic Regions of India | Zendy

C. Appunu | Zendy; Angèle N’Zoué | Zendy; Gisèle Laguerre | Zendy

Open Access

Genetic Diversity of Native Bradyrhizobia Isolated from Soybeans ( Glycine max L.) in Different Agricultural-Ecological-Climatic Regions of India

Author(s) -

C. Appunu,

Angèle N’Zoué,

Gisèle Laguerre

Publication year - 2008

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01320-08

Subject(s) - biology , glycine , agriculture , genetic diversity , ecology , diversity (politics) , botany , genetics , population , demography , amino acid , sociology , anthropology

Fifty isolates from root nodules of soybean plants sampled in five agricultural-ecological-climatic regions of India were analyzed by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene, the intergenic spacer region between the 16S and 23S rRNA genes (IGS), and thenifH andnodC genes. Eight haplotypes assigned to theBradyrhizobium genus were identified, and the genetic diversity was conserved across regions. Sequence analyses of the IGS and thednaK, glnII, recA , andnifH genes revealed three groups. One of them (26% of isolates) was assigned toBradyrhizobium liaoningense . A second group (36% of isolates) was identified asB. yuanmingense but likely forms a new biovar able to nodulate soybean plants. The third lineage (38% of isolates) was different from all describedBradyrhizobium species but showed the same symbiotic genotype asB. liaoningense andB. japonicum bv.glycinearum .

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