Ectoine Biosynthesis in Mycobacterium smegmatis | Zendy

Naomi Ofer | Zendy; Marina Wishkautzan | Zendy; Michaël M. Meijler | Zendy; Ying Wang | Zendy; Alexander Speer | Zendy; Michael Niederweis | Zendy; Eyal Gur | Zendy

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Ectoine Biosynthesis in Mycobacterium smegmatis

Author(s) -

Naomi Ofer,

Marina Wishkautzan,

Michaël M. Meijler,

Ying Wang,

Alexander Speer,

Michael Niederweis,

Eyal Gur

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01318-12

Subject(s) - mycobacterium smegmatis , ectoine , mutant , biology , phenotype , biosynthesis , mycobacterium , halotolerance , microbiology and biotechnology , biochemistry , bacteria , genetics , gene , computational biology , osmoprotectant , mycobacterium tuberculosis , amino acid , medicine , tuberculosis , pathology , proline

Mycobacterium smegmatis is a commonly used mycobacterial model system. Here, we show that M. smegmatis protects itself against elevated salinity by synthesizing ectoine and hydroxyectoine and characterize the phenotype of a nonproducing mutant. This is the first analysis of M. smegmatis halotolerance and of the molecular mechanism that supports it.

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