Open AccessEnhancement of Recombinant Hemoglobin Production in Escherichia coli BL21(DE3) Containing the Plesiomonas shigelloides Heme Transport System
Author(s) -
Donaldo Villarreal,
Cynthia L. Phillips,
Angela Kelley,
S. Villarreal,
A. Villaloboz,
Pablo E. Hernández,
John S. Olson,
Douglas P. Henderson
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01291-08
Subject(s) - plesiomonas shigelloides , escherichia coli , recombinant dna , heme , hemoglobin , biochemistry , microbiology and biotechnology , chemistry , biology , enterobacteriaceae , bacteria , gene , enzyme , genetics
To produce recombinant hemoglobin inEscherichia coli , sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes andPlesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production inE. coli BL21(DE3) grown in medium containing heme.