Global Analysis of Furfural-Induced Genomic Instability Using a Yeast Model

Whether and how furfural affects genome integrity have not been clarified. Using aSaccharomyces cerevisiae model, we found that furfural exposure leads toin vivo DSBs and elevation in mitotic recombination by orders of magnitude. Gross chromosomal rearrangements and aneuploidy events also occurred at a higher frequency in furfural-treated cells. In a genome-wide analysis, we show that the patterns of mitotic recombination and point mutations differed dramatically in furfural-treated cells and wild-type cells.