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Mutation of a novelcry -like gene (cry256 ) fromBacillus thuringiensis resulted in a protein crystal, normally located within the spore's exosporium, being found predominately outside the exosporium. Thecry256 gene codes for a 3-domain Cry-like protein that does not correspond to any of the known Cry protein holotypes.

A Novel Bacillus thuringiensis Cry-Like Protein from a Rare Filamentous Strain Promotes Crystal Localization within the Exosporium