Regulatable Vectors for Environmental Gene Expression in Alphaproteobacteria | Zendy

Adrian Tett | Zendy; Steven Rudder | Zendy; Alexandre Bourdï¿ ⁄ s | Zendy; Ramakrishnan Karunakaran | Zendy; Philip S. Poole | Zendy

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Regulatable Vectors for Environmental Gene Expression in Alphaproteobacteria

Author(s) -

Adrian Tett,

Steven Rudder,

Alexandre Bourdï¿ ⁄ s,

Ramakrishnan Karunakaran,

Philip S. Poole

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01188-12

Subject(s) - cloning (programming) , plasmid , biology , expression vector , vector (molecular biology) , cloning vector , genetics , gene , gene expression , alphaproteobacteria , computational biology , molecular cloning , multiple cloning site , microbiology and biotechnology , recombinant dna , computer science , 16s ribosomal rna , programming language

Two expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.

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