Stable, Site-Specific Fluorescent Tagging Constructs Optimized for Burkholderia Species | Zendy

Michael H. Norris | Zendy; Yun Kang | Zendy; Bruce A. Wilcox | Zendy; Tung T. Hoang | Zendy

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Stable, Site-Specific Fluorescent Tagging Constructs Optimized for Burkholderia Species

Author(s) -

Michael H. Norris,

Yun Kang,

Bruce A. Wilcox,

Tung T. Hoang

Publication year - 2010

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01188-10

Subject(s) - fluorescence , biology , burkholderia , computational biology , fluorescent protein , green fluorescent protein , bacteria , genetics , physics , quantum mechanics , gene

Several vectors that facilitate stable fluorescent labeling ofBurkholderia pseudomallei andBurkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized forBurkholderia spp., enabling bacterial tracking during cellular infection.

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