Open AccessStable, Site-Specific Fluorescent Tagging Constructs Optimized for Burkholderia Species
Author(s) -
Michael H. Norris,
Yun Kang,
Bruce A. Wilcox,
Tung T. Hoang
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01188-10
Subject(s) - fluorescence , biology , burkholderia , computational biology , fluorescent protein , green fluorescent protein , bacteria , genetics , physics , quantum mechanics , gene
Several vectors that facilitate stable fluorescent labeling ofBurkholderia pseudomallei andBurkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized forBurkholderia spp., enabling bacterial tracking during cellular infection.