Effective Soil Extraction Method for Cultivating Previously Uncultured Soil Bacteria | Zendy

Tuan Manh Nguyen | Zendy; Chan Seo | Zendy; Moongi Ji | Zendy; ManJeong Paik | Zendy; SeungWoon Myung | Zendy; Jaisoo Kim | Zendy

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Effective Soil Extraction Method for Cultivating Previously Uncultured Soil Bacteria

Author(s) -

Tuan Manh Nguyen,

Chan Seo,

Moongi Ji,

ManJeong Paik,

SeungWoon Myung,

Jaisoo Kim

Publication year - 2018

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01145-18

Subject(s) - soil bacteria , bacteria , soil microbiology , extraction (chemistry) , biology , environmental science , chemistry , chromatography , genetics

Both metagenomics and single-cell sequencing can detect unknown genes from uncultured microbial strains in environments, and either method may find the significant potential metabolites and roles of these strains. However, such gene/genome-based techniques do not allow detailed investigations that are possible with cultures. To solve this problem, various approaches for cultivation of uncultured bacteria have been developed, but there are still difficulties in maintaining pure cultures by subculture.

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