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Cell proliferation and alpha-toxin gene expression of Clostridium perfringens in relation to the development of necrotic enteritis (NE) were investigated. Unlike bacitracin-treated chickens, non-bacitracin-treated birds exhibited typical NE symptoms and reduced growth performance. They also demonstrated increased C. perfringens proliferation and alpha-toxin gene expression that were positively correlated and progressed according to the regression model y = b(0) + b(1)X - b(2)X(2). The average C. perfringens count of 5 log(10) CFU/g in the ileal digesta appears to be a threshold for developing NE with a lesion score of 2.

applied and environmental microbiology

Quantification of Cell Proliferation and Alpha-Toxin Gene Expression of<i>Clostridium perfringens</i>in the Development of Necrotic Enteritis in Broiler Chickens

Quantification of Cell Proliferation and Alpha-Toxin Gene Expression ofClostridium perfringensin the Development of Necrotic Enteritis in Broiler Chickens