Open AccessEvaluating the Insecticidal Genes and Their Expressed Products in Bacillus thuringiensis Strains by Combining PCR with Mass Spectrometry
Author(s) -
Yunjun Sun,
Zihao Fu,
Xuezhi Ding,
Liqiu Xia
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01085-08
Subject(s) - bacillus thuringiensis , biology , bacillales , gene , bacillaceae , mass spectrometry , microbiology and biotechnology , genetics , bacteria , chemistry , chromatography , bacillus subtilis
By a combination of PCR and mass spectrometry, a total of fivecry genes (cry1Aa, cry1Ac, cry2Aa, cry2Ab , andcry1Ia ) were detected in genomic DNA from the wild-typeBacillus thuringiensis strain 4.0718, and three protoxins (Cry1Aa, Cry1Ac, and Cry2Aa) were identified in the strain's parasporal crystals. These results indicated that this complementary method may be useful in evaluatingB. thuringiensis strains at both the gene and protein levels.