Inactivation of the ybdD Gene in Lactococcus lactis Increases the Amounts of Exported Proteins | Zendy

Eric Morello | Zendy; Sébastien Nouaille | Zendy; Naima G. CortesPerez | Zendy; Sébastien Blugeon | Zendy; L.F.C. Medina | Zendy; Vasco Azevedo | Zendy; JeanJacques Gratadoux | Zendy; L. G. Bermï¿ ⁄ dez-Humarï¿ ⁄ n | Zendy; Yves Le Loir | Zendy; Philippe Langella | Zendy

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Inactivation of the ybdD Gene in Lactococcus lactis Increases the Amounts of Exported Proteins

Author(s) -

Eric Morello,

Sébastien Nouaille,

Naima G. CortesPerez,

Sébastien Blugeon,

L.F.C. Medina,

Vasco Azevedo,

JeanJacques Gratadoux,

L. G. Bermï¿ ⁄ dez-Humarï¿ ⁄ n,

Yves Le Loir,

Philippe Langella

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01076-12

Subject(s) - lactococcus lactis , insertional mutagenesis , heterologous , mutagenesis , mutant , biology , gene , plasmid , genetics , bacteria , lactic acid

Random insertional mutagenesis performed on a Lactococcus lactis reporter strain led us to identify L. lactis ybdD as a protein-overproducing mutant. In different expression contexts, the ybdD mutant shows increased levels of exported proteins and therefore constitutes a new and attractive heterologous protein production host. This study also highlights the importance of unknown regulatory processes that play a role during protein secretion.

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