Open AccessRole of the ptsN Gene Product in Catabolite Repression of the Pseudomonas putida TOL Toluene Degradation Pathway in Chemostat Cultures ▿
Author(s) -
Isabel Aranda-Olmedo,
Patricia Marín,
Juan L. Ramos,
Silvia Marqués
Publication year - 2006
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.01067-06
Subject(s) - catabolite repression , pseudomonas putida , chemostat , psychological repression , fed batch culture , pseudomonas , toluene , chemistry , biochemistry , yeast , biology , gene , bacteria , gene expression , mutant , fermentation , organic chemistry , genetics
The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIA(Ntr) participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influence expression when cells were growing in a chemostat with succinate at maximum rate.