Molecular Biological Detection of Anaerobic Gut Fungi ( Neocallimastigales ) from Landfill Sites | Zendy

Robert J. Lockhart | Zendy; Michele I. Van Dyke | Zendy; I. R. Beadle | Zendy; Paul Humphreys | Zendy; Alan J. McCarthy | Zendy

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Molecular Biological Detection of Anaerobic Gut Fungi ( Neocallimastigales ) from Landfill Sites

Author(s) -

Robert J. Lockhart,

Michele I. Van Dyke,

I. R. Beadle,

Paul Humphreys,

Alan J. McCarthy

Publication year - 2006

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01057-06

Subject(s) - biology , phylogenetic tree , oligomer restriction , environmental dna , microbiology and biotechnology , polymerase chain reaction , nested polymerase chain reaction , ribosomal rna , gene , microbial ecology , oligonucleotide , clone (java method) , computational biology , genetics , ecology , bacteria , biodiversity

Oligonucleotide primers were designed for the 18S rRNA genes of members of the Neocallimastigales and used in a nested PCR protocol to amplify 787-bp fragments of DNA from landfill site samples. The specificities of the primers were confirmed by phylogenetic analysis of the environmental clone sequences, and this method can therefore now be used to investigate the ecology of the obligately anaerobic fungi. To our knowledge, this is the first demonstration of the occurrence of members of the Neocallimastigales outside the mammalian gut, and their distribution across the landfill samples examined here suggests that they are actively involved in cellulose degradation.

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