Simple and Safe Method for Simultaneous Isolation of Microbial RNA and DNA from Problematic Populations | Zendy

Simon Jon McIlroy | Zendy; Kate Porter | Zendy; Robert J. Seviour | Zendy; Daniel Tillett | Zendy

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Simple and Safe Method for Simultaneous Isolation of Microbial RNA and DNA from Problematic Populations

Author(s) -

Simon Jon McIlroy,

Kate Porter,

Robert J. Seviour,

Daniel Tillett

Publication year - 2008

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01047-08

Subject(s) - nucleic acid , dna , rna , dna extraction , isolation (microbiology) , biology , rna extraction , nucleic acid methods , chloroform , extraction (chemistry) , computational biology , chromatography , microbiology and biotechnology , chemistry , genetics , polymerase chain reaction , gene

We describe a novel, rapid, and safe method for extracting RNA and DNA from refractory microbes, which avoids the use of phenol or chloroform. It has been used successfully to isolate high-quality nucleic acids from pure cultures and environmental populations known to resist widely used extraction protocols.

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