A Targeted Multilocus Genotyping Assay for Lineage, Serogroup, and Epidemic Clone Typing of Listeria monocytogenes | Zendy

Todd J. Ward | Zendy; Thomas Usgaard | Zendy; Peter S. Evans | Zendy

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A Targeted Multilocus Genotyping Assay for Lineage, Serogroup, and Epidemic Clone Typing of Listeria monocytogenes

Author(s) -

Todd J. Ward,

Thomas Usgaard,

Peter S. Evans

Publication year - 2010

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.01008-10

Subject(s) - listeria monocytogenes , genotyping , clone (java method) , typing , multilocus sequence typing , biology , subtyping , lineage (genetic) , microbiology and biotechnology , virology , listeria , serotype , genotype , genetics , bacteria , gene , computer science , programming language

A 30-probe assay was developed for simultaneous classification of Listeria monocytogenes isolates by lineage (I to IV), major serogroup (4b, 1/2b, 1/2a, and 1/2c), and epidemic clone (EC) type (ECI, ECIa, ECII, and ECIII). The assay was designed to facilitate rapid strain characterization and the integration of subtype data into risk-based inspection programs.

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