Open AccessInterdomain Conjugal Transfer of DNA from Bacteria to Archaea
Author(s) -
Jeremy A. Dodsworth,
Lei Li,
Shiping Wei,
Brian P. Hedlund,
John A. Leigh,
Paul de Figueiredo
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00967-10
Subject(s) - methanococcus , archaea , bacteria , escherichia coli , biology , transformation (genetics) , methanosarcina barkeri , genetics , dna , horizontal gene transfer , microbiology and biotechnology , genome , gene , methanogenesis
Escherichia coli transforms the methanogenic archaeonMethanococcus maripaludis at frequencies ranging from 0.2 × 10−6 to 2 × 10−6 per recipient cell. Transformation requires cell-to-cell contact,oriT , andtra functions, is insensitive to DNase I, and otherwise displays hallmarks of conjugation.