Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability | Zendy

Wenjin Liu | Zendy; Xiaozhou Zhang | Zendy; Zuoming Zhang | Zendy; Y.H. Percival Zhang | Zendy

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Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability

Author(s) -

Wenjin Liu,

Xiaozhou Zhang,

Zuoming Zhang,

Y.H. Percival Zhang

Publication year - 2010

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00958-10

Subject(s) - cellulase , thermostability , cellulose , glycoside hydrolase , mutant , escherichia coli , carboxymethyl cellulose , chemistry , cellulosic ethanol , biochemistry , hydrolase , cell wall , enzyme , gene , organic chemistry , sodium

A family 5 glycoside hydrolase fromClostridium phytofermentans was cloned and engineered through a cellulase cell surface display system inEscherichia coli . The presence of cell surface anchoring, a cellulose binding module, or a His tag greatly influenced the activities of wild-type and mutant enzymes on soluble and solid cellulosic substrates, suggesting the high complexity of cellulase engineering. The best mutant had 92%, 36%, and 46% longer half-lives at 60°C on carboxymethyl cellulose, regenerated amorphous cellulose, and Avicel, respectively.

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