Open AccessDevelopment of a Real-Time qPCR Method for Detection and Enumeration of Mycobacterium spp. in Surface Water
Author(s) -
Nicolas Radomski,
Françoise S. Lucas,
Régis Moilleron,
Emmanuelle Cambau,
Sophie Haenn,
Laurent Moulin
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00942-10
Subject(s) - enumeration , 16s ribosomal rna , biology , mycobacterium , real time polymerase chain reaction , ribosomal rna , microbiology and biotechnology , bacteria , computational biology , gene , genetics , mathematics , combinatorics
A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively).
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