Mutations That Disrupt Either thepqqor thegdhGene ofRahnella aquatilisAbolish the Production of an Antibacterial Substance and Result in Reduced Biological Control of Grapevine Crown Gall

Rahnella aquatilis HX2, a biocontrol agent for grapevine crown gall caused byAgrobacterium vitis , produces an antibacterial substance that inhibits the growth ofA. vitis in vitro. In this study, we show that MH15 and MH16, two Tn5 -induced mutants of HX2, have lost their abilities to inhibitA. vitis and have reduced biocontrol activities; they grow in logarithmic phase at a rate similar to that of the wild type and have single Tn5 insertions. They are also impaired in producing pyrroloquinoline quinone (PQQ) or glucose dehydrogenase (GDH). Complementation of MH15 and MH16 with cosmid clones of CP465 and CP104 from an HX2 DNA library restored the antibiosis, biocontrol, and PQQ or GDH production phenotypes. A 6.7-kb BamHI fragment from CP465 that fully restored the MH15-affected phenotypes was cloned and sequenced. Sequence analysis of the mutated DNA region resulted in the identification of seven open reading frames (ORFs), six of which share significant homology with PQQ-synthesizing genes in other bacteria, designatedpqqA throughpqqF . Meanwhile, A 5.5-kb PstI fragment from CP104 fully complemented the MH16 mutant and contained a single ORF highly similar to that of genes coding for GDHs. An in-framegdh deletion mutant has the same phenotypes as the Tn5 mutant of MH16. Complementation of both deletion and Tn5 gdh mutants restored the affected phenotypes to wild-type levels. Our results suggest that an antibacterial substance plays a role in biocontrol ofA. vitis by HX2.