Open AccessCharacterization of a Family GH5 Xylanase with Activity on Neutral Oligosaccharides and Evaluation as a Pulp Bleaching Aid
Author(s) -
Óscar Gallardo,
María FernándezFernández,
Cristina Valls,
Susana V. Valenzuela,
M. Blanca Roncero,
Teresa Vidal,
Pilar Dı́az,
F. I. Javier Pastor
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00871-10
Subject(s) - xylanase , kraft process , chemistry , pulp (tooth) , kraft paper , glycosyl , enzyme , food science , biochemistry , organic chemistry , pulp and paper industry , medicine , pathology , engineering
A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B fromBacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for methyl-glucuronic acid side chains for catalysis. The enzyme was evaluated onEucalyptus kraft pulp, showing its effectiveness as a bleaching aid.