Zirex: a Novel Zinc-Regulated Expression System for Lactococcus lactis | Zendy

Dongdong Mu | Zendy; Manuel Montalbï¿ ⁄ n-Lï¿ ⁄ pez | Zendy; Yoshimitsu Masuda | Zendy; Oscar P. Kuipers | Zendy

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Zirex: a Novel Zinc-Regulated Expression System for Lactococcus lactis

Author(s) -

Dongdong Mu,

Manuel Montalbï¿ ⁄ n-Lï¿ ⁄ pez,

Yoshimitsu Masuda,

Oscar P. Kuipers

Publication year - 2013

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00866-13

Subject(s) - lactococcus lactis , repressor , nisin , biology , zinc , zinc finger , gene , gene expression , chemistry , microbiology and biotechnology , computational biology , bacteria , biochemistry , genetics , transcription factor , lactic acid , organic chemistry

Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and PczcD. PczcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes.

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