One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies | Zendy

Jae-Yeon Jeong | Zendy; HyungSoon Yim | Zendy; Ji-Young Ryu | Zendy; Hyun Sook Lee | Zendy; Jung-Hyun Lee | Zendy; DongSeung Seen | Zendy; Sung Gyun Kang | Zendy

Open Access

One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies

Author(s) -

Jae-Yeon Jeong,

HyungSoon Yim,

Ji-Young Ryu,

Hyun Sook Lee,

Jung-Hyun Lee,

DongSeung Seen,

Sung Gyun Kang

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00844-12

Subject(s) - cloning (programming) , transformation (genetics) , cloning vector , ligation , computational biology , insert (composites) , molecular cloning , biology , genetics , microbiology and biotechnology , computer science , gene , peptide sequence , materials science , composite material , programming language

We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research