Open AccessActinobacterial Community Structure in Soils Receiving Long-Term Organic and Inorganic Amendments
Author(s) -
Zhe Piao,
Linzhang Yang,
Liping Zhao,
Shixue Yin
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00843-07
Subject(s) - temperature gradient gel electrophoresis , phylogenetic diversity , microbial population biology , community structure , soil water , biology , 16s ribosomal rna , agronomy , ecology , phylogenetic tree , bacteria , biochemistry , genetics , gene
The impact of long-term organic and inorganic amendments on the actinobacterial community in soils was studied. Denaturing gradient gel electrophoresis patterns based on the V3 region of 16S rRNA suggested that there was no significant difference between the communities occurring in the different amendments. However, analysis of the clone libraries of the actinobacterial communities by the use of multiple statistical approaches showed that these communities were significantly different from each other. Results showed that long-term organic and inorganic soil amendments did not significantly alter the overall phylogenetic diversity of the actinobacterial communities but did significantly change the community structure.