A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an IS 3 Family Insertion Sequence, IS Blo11 | Zendy

Mikiyasu Sakanaka | Zendy; Shingo Nakakawaji | Zendy; Shin Nakajima | Zendy; Satoru Fukiya | Zendy; Arisa Abe | Zendy; Wataru Saburi | Zendy; Haruhide Mori | Zendy; Atsushi Yokota | Zendy

Open Access

A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an IS 3 Family Insertion Sequence, IS Blo11

Author(s) -

Mikiyasu Sakanaka,

Shingo Nakakawaji,

Shin Nakajima,

Satoru Fukiya,

Arisa Abe,

Wataru Saburi,

Haruhide Mori,

Atsushi Yokota

Publication year - 2018

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00824-18

Subject(s) - bifidobacterium longum , transposon mutagenesis , biology , transposable element , mutagenesis , bifidobacterium , genetics , gene , microbiology and biotechnology , bacteria , genome , mutation , lactobacillus

Several hundred species of bacteria colonize the mammalian intestine. However, the genes that enable such bacteria to colonize and thrive in the intestine remain largely unexplored. Transposon mutagenesis, combined with next-generation sequencing, is a promising tool to comprehensively identify these genes but has so far been applied only to a small number of intestinal bacterial species. In this study, a transposon mutagenesis system was established forBifidobacterium longum subsp.longum , a representative health-promotingBifidobacterium species. The system enables the identification of genes that promote colonization and survival in the intestine and should help illuminate the physiology of this species.

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