Single-Homology-Arm Linear DNA Recombination by the Nonhomologous End Joining Pathway as a Novel and Simple Gene Inactivation Method: a Proof-of-Concept Study in Dietzia sp. Strain DQ12-45-1b | Zendy

Shelian Lu | Zendy; Yong Nie | Zendy; Meng Wang | Zendy; Hongxiu Xu | Zendy; Dong-Ling Ma | Zendy; JieLiang Liang | Zendy; XiaoLei Wu | Zendy

Open Access

Single-Homology-Arm Linear DNA Recombination by the Nonhomologous End Joining Pathway as a Novel and Simple Gene Inactivation Method: a Proof-of-Concept Study in Dietzia sp. Strain DQ12-45-1b

Author(s) -

Shelian Lu,

Yong Nie,

Meng Wang,

Hongxiu Xu,

Dong-Ling Ma,

JieLiang Liang,

XiaoLei Wu

Publication year - 2018

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00795-18

Subject(s) - biology , plasmid , dna ligase , genetics , genome , dna , gene , homology (biology) , genome editing , circular bacterial chromosome , non homologous end joining , in vitro recombination , homologous recombination , computational biology , molecular cloning , peptide sequence

Many nonmodel Gram-positive bacteria lack efficient genetic manipulation systems, but they express genes encoding Ku and LigD. The NHEJ pathway inDietzia sp. DQ12-45-1b was evaluated and was used to successfully knock out 11 genes in the genome. Since bioinformatic studies revealed that the putative genes encoding Ku and LigD ubiquitously exist in phylogenetically diverse bacteria and archaea, the single-homology-arm linear DNA recombination by the NHEJ pathway could be a potentially applicable genetic manipulation method for diverse nonmodel prokaryotic organisms.

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