English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Coculturing dark- and photofermentative bacteria is a promising strategy for enhanced hydrogen (H2 ) production. In this study, next-generation sequencing was used to query the global transcriptomic responses of an artificial coculture ofClostridium cellulovorans 743B andRhodopseudomonas palustris CGA009. By analyzing differentially regulated gene expression, we showed that, consistent with the physiological observations of enhanced H2 production and cellulose degradation, the nitrogen fixation genes inR. palustris and the cellulosomal genes inC. cellulovorans were upregulated in cocultures. Unexpectedly, genes related to H2 production inC. cellulovorans were downregulated, suggesting that the enhanced H2 yield was contributed mainly byR. palustris . A number of genes related to biosynthesis of volatile fatty acids (VFAs) inC. cellulovorans were upregulated, and correspondingly, a gene that mediates organic compound catabolism inR. palustris was also upregulated. Interestingly, a number of genes responsible for chemotaxis inR. palustris were upregulated, which might be elicited by the VFA concentration gradient created byC. cellulovorans . In addition, genes responsible for sulfur and thiamine metabolism inC. cellulovorans were downregulated in cocultures, and this could be due to a response to pH changes. A conceptual model illustrating the interactions between the two organisms was constructed based on the transcriptomic results.IMPORTANCE The findings of this study have important biotechnology applications for biohydrogen production using renewable cellulose, which is an industrially and economically important bioenergy process. Since the molecular characteristics of the interactions of a coculture when cellulose is the substrate are still unclear, this work will be of interest to microbiologists seeking to better understand and optimize hydrogen-producing coculture systems.

applied and environmental microbiology

Transcriptomic Responses of the Interactions between Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009 in a Cellulose-Grown Coculture for Enhanced Hydrogen Production