Open Access6-Aminohexanoate Oligomer Hydrolases from the Alkalophilic Bacteria Agromyces sp. Strain KY5R and Kocuria sp. Strain KY2
Author(s) -
Kengo Yasuhira,
Yasuhito Tanaka,
Hiroshi Shibata,
Yosuke Kawashima,
Akira Ohara,
Daiichiro Kato,
Masahiro Takeo,
Seiji Negoro
Publication year - 2007
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00777-07
Subject(s) - thermostability , oligomer , bacteria , strain (injury) , arthrobacter , microbiology and biotechnology , biology , chemistry , enzyme , biochemistry , polymer chemistry , genetics , anatomy
Alkalophilic, nylon oligomer-degrading strains,Agromyces sp. andKocuria sp., were isolated from the wastewater of a nylon-6 factory and from activated sludge from a sewage disposal plant. The 6-aminohexanoate oligomer hydrolases (NylC) from the alkalophilic strains had 95.8 to 98.6% similarity to the enzyme in neutrophilicArthrobacter sp. but had superior thermostability, activity under alkaline conditions, and affinity for nylon-related substrates, which would be advantageous for biotechnological applications.