Antibiotic Resistance Markers for Genetic Manipulations of Leptospira spp | Zendy

Dante Poggi | Zendy; P.O. Giuseppe | Zendy; Mathieu Picardeau | Zendy

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Antibiotic Resistance Markers for Genetic Manipulations of Leptospira spp

Author(s) -

Dante Poggi,

P.O. Giuseppe,

Mathieu Picardeau

Publication year - 2010

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00775-10

Subject(s) - spectinomycin , kanamycin , leptospira , streptomycin , biology , selectable marker , microbiology and biotechnology , gentamicin , genetics , gene , antibiotic resistance , antibiotics , plasmid , serotype

We measured the frequency of appearance of spontaneous mutants resistant to gentamicin, kanamycin, streptomycin, and spectinomycin in saprophytic and pathogenicLeptospira strains. The mutations responsible for the spontaneous resistance to streptomycin and spectinomycin were identified in therpsL andrrs genes, respectively. We also generated a gentamicin resistance cassette that allows the use of a third selectable marker in leptospires. These results may facilitate further advances in gene transfer systems inLeptospira spp.

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