Production of Lactic Acid from Cellobiose and Cellotriose by Lactobacillus delbrueckii Mutant Uc-3
Author(s) -
Mukund Adsul,
J. M. Khire,
K. B. Bastawde,
D. V. Gokhale
Publication year - 2007
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00774-07
Subject(s) - cellobiose , lactic acid , lactobacillus , mutant , biochemistry , lactobacillaceae , fermentation , chemistry , bacteria , enzyme , biology , food science , cellulase , gene , genetics
Lactobacillus delbrueckii mutant Uc-3 utilizes both cellobiose and cellotriose efficiently, converting it into L(+) lactic acid. The enzyme activities of cellobiose and cellotriose utilization were determined for cell extracts, whole cells, and disrupted cells. Aryl-beta-glucosidase activity was detected only for whole cells and disrupted cells, suggesting that these activities are cell bound. The mutant produced 90 g/liter of lactic acid from 100 g/liter of cellobiose with 2.25 g/liter/h productivity.
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