Open AccessIsolation from Ochrobactrum anthropi of a Novel Class II 5-Enopyruvylshikimate-3-Phosphate Synthase with High Tolerance to Glyphosate
Author(s) -
YongSheng Tian,
AiSheng Xiong,
Jing Xu,
Zhao Wang,
Feng Gao,
Xin Fu,
Huini Xu,
Jinhai Zheng,
RiHe Peng,
QuanHong Yao
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00770-10
Subject(s) - glyphosate , isolation (microbiology) , biology , microbiology and biotechnology , atp synthase , bacteria , chemistry , biochemistry , genetics , gene
Applying the genomic library construction process and colony screening, a novelaro A gene encoding 5-enopyruvylshikimate-3-phosphate synthase fromOchrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance.