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A Novel Method for Sampling and Long-Term Monitoring of Microbes That Uses Stickers of Plain Paper
Author(s) -
Martin Bobal,
Anna Kristina Witte,
Patrick Mester,
Susanne Fister,
Dagmar Schoder,
Peter Rossmanith
Publication year - 2019
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00766-19
Subject(s) - sampling (signal processing) , listeria monocytogenes , bacteria , term (time) , biology , computer science , biochemical engineering , engineering , telecommunications , physics , quantum mechanics , genetics , detector
As a ubiquitous bacterium,Listeria monocytogenes has a propensity to enter food production areas inadvertently via fomites such as door handles and switches. While the bacterium might not be in direct contact with the food products, knowing the microbial status of the surroundings is essential for risk assessment. Our investigation into a novel quantitative PCR (qPCR)-based sampling system with the highest sensitivity and ability to monitor over long periods of time, yet based on paper, proved to be cost-effective and reasonably convenient to handle.

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