Scaffoldin Modules Serving as “Cargo” Domains To Promote the Secretion of Heterologous Cellulosomal Cellulases by Clostridium acetobutylicum | Zendy

Angélique Chanal | Zendy; Florence Mingardon | Zendy; Marielle Bauzan | Zendy; Chantal Tardif | Zendy; Henri-Pierre Fiérobe | Zendy

Open Access

Scaffoldin Modules Serving as “Cargo” Domains To Promote the Secretion of Heterologous Cellulosomal Cellulases by Clostridium acetobutylicum

Author(s) -

Angélique Chanal,

Florence Mingardon,

Marielle Bauzan,

Chantal Tardif,

Henri-Pierre Fiérobe

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00758-11

Subject(s) - clostridium acetobutylicum , cellulase , heterologous , secretion , clostridium , microbiology and biotechnology , bacteria , cellulose , clostridium thermocellum , clostridiaceae , biology , heterologous expression , biochemistry , chemistry , recombinant dna , gene , genetics , butanol , ethanol , toxin

The secretion of large heterologous cellulases by Clostridium acetobutylicum was formerly shown to be deleterious. To circumvent this issue, various scaffoldins' modules were grafted at their N termini. Family 3a cellulose binding module combined with an X2 module(s) was found to trigger the secretion of Clostridium cellulolyticum cellulases by the solventogenic bacterium.

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