Detection of Mycobacterium avium subsp. paratuberculosis in Drinking Water and Biofilms by Quantitative PCR | Zendy

Amy Beumer | Zendy; Dawn King | Zendy; Maura J. Donohue | Zendy; Jatin H. Mistry | Zendy; Terry C. Covert | Zendy; Stacy Pfaller | Zendy

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Detection of Mycobacterium avium subsp. paratuberculosis in Drinking Water and Biofilms by Quantitative PCR

Author(s) -

Amy Beumer,

Dawn King,

Maura J. Donohue,

Jatin H. Mistry,

Terry C. Covert,

Stacy Pfaller

Publication year - 2010

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00730-10

Subject(s) - paratuberculosis , mycobacterium avium subspecies paratuberculosis , mycobacterium avium subsp. paratuberculosis , microbiology and biotechnology , mycobacterium , biology , biofilm , organism , polymerase chain reaction , bacteria , virology , gene , genetics

It has been suggested that Mycobacterium avium subspecies paratuberculosis has a role in Crohn's disease. The organism may be acquired but is difficult to culture from the environment. We describe a quantitative PCR (qPCR) method to detect M. avium subsp. paratuberculosis in drinking water and the results of its application to drinking water and faucet biofilm samples collected in the United States.

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