Quantitative PCR for Tracking the Megaplasmid-Borne Biodegradation Potential of a Model Sphingomonad | Zendy

Erica M. Hartmann | Zendy; Jonathan P. Badalamenti | Zendy; Rosa KrajmalnikBrown | Zendy; Rolf U. Halden | Zendy

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Quantitative PCR for Tracking the Megaplasmid-Borne Biodegradation Potential of a Model Sphingomonad

Author(s) -

Erica M. Hartmann,

Jonathan P. Badalamenti,

Rosa KrajmalnikBrown,

Rolf U. Halden

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00715-12

Subject(s) - dioxygenase , biodegradation , biology , sphingomonas , gene , plasmid , degradation (telecommunications) , bioreactor , bacteria , microbiology and biotechnology , pseudomonas , genetics , ecology , botany , telecommunications , computer science

We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenase-encoding genes.

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