Molecular Diversity, Cultivation, and Improved Detection by Fluorescent In Situ Hybridization of a Dominant Group of Human Gut Bacteria Related to Roseburia spp. or Eubacterium rectale | Zendy

Rustam Aminov | Zendy; Alan W. Walker | Zendy; Sylvia H. Duncan | Zendy; Hermie J. M. Harmsen | Zendy; Gjalt W. Welling | Zendy; Harry J. Flint | Zendy

Open Access

Molecular Diversity, Cultivation, and Improved Detection by Fluorescent In Situ Hybridization of a Dominant Group of Human Gut Bacteria Related to Roseburia spp. or Eubacterium rectale

Author(s) -

Rustam Aminov,

Alan W. Walker,

Sylvia H. Duncan,

Hermie J. M. Harmsen,

Gjalt W. Welling,

Harry J. Flint

Publication year - 2006

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00701-06

Subject(s) - biology , phylotype , roseburia , eubacterium , oligomer restriction , microbiology and biotechnology , bacteria , 16s ribosomal rna , genetics , fluorescence in situ hybridization , phylogenetic tree , oligonucleotide , dna , gene , chromosome , bacteroides

Phylogenetic analysis was used to compare 16S rRNA sequences from 19 cultured human gut strains of Roseburia and Eubacterium rectale with 356 related sequences derived from clone libraries. The cultured strains were found to represent five of the six phylotypes identified. A new oligonucleotide probe, Rrec584, and the previous group probe Rint623, when used in conjunction with a new helper oligonucleotide, each recognized an average of 7% of bacteria detected by the eubacterial probe Eub338 in feces from 10 healthy volunteers. Most of the diversity within this important group of butyrate-producing gut bacteria can apparently be retrieved through cultivation.

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