Identification and Molecular Characterization of Class 1 Integrons in Multiresistant Escherichia coli Isolates from Poultry Litter | Zendy

Elizabeth PonceRivas | Zendy; MaríaEnriqueta MuñozMárquez | Zendy; Ashraf A. Khan | Zendy

Open Access

Identification and Molecular Characterization of Class 1 Integrons in Multiresistant Escherichia coli Isolates from Poultry Litter

Author(s) -

Elizabeth PonceRivas,

MaríaEnriqueta MuñozMárquez,

Ashraf A. Khan

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00660-12

Subject(s) - integron , biology , pulsed field gel electrophoresis , plasmid , escherichia coli , microbiology and biotechnology , genetics , gene , streptomycin , gene cassette , antibiotic resistance , antibiotics , genotype

This study describes the prevalence of arrays of class 1 integron cassettes and Qnr determinants (A, B, and S) in 19 fluoroquinolone-resistantEscherichia coli isolates from chicken litter.qnrS andqnrA were the predominant genes in these fluoroquinolone-resistant isolates, and an uncommon array ofaacA4 -catB3 -dfrA1 gene cassettes from a class1 integron was found. Additionally,aadA1 anddfrA1 gene cassettes, encoding resistance to streptomycin and trimethoprim, constituted the most common genes identified and was located on megaplasmids as well on the chromosome. Antibiotic resistance, pulsed-field gel electrophoresis (PFGE), and plasmid data suggest a genetically diverse origin of poultryE. coli isolates.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research