Open AccessDevelopment of a Two-Color Fluorescence In Situ Hybridization Technique for Species-Level Identification of Human-Infectious Cryptosporidium spp
Author(s) -
Anitha Alagappan,
Peter L. Bergquist,
Belinda C. Ferrari
Publication year - 2009
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00643-09
Subject(s) - cryptosporidium , cryptosporidium parvum , biology , fluorescence in situ hybridization , in situ , identification (biology) , fluorescence , microbiology and biotechnology , virology , computational biology , genetics , chemistry , ecology , feces , gene , optics , organic chemistry , chromosome , physics
A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification ofCryptosporidium parvum andC. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the majorCryptosporidium species of concern to public health.