Development of a Two-Color Fluorescence In Situ Hybridization Technique for Species-Level Identification of Human-Infectious Cryptosporidium spp | Zendy

A. Alagappan | Zendy; Peter L. Bergquist | Zendy; Belinda C. Ferrari | Zendy

Open Access

Development of a Two-Color Fluorescence In Situ Hybridization Technique for Species-Level Identification of Human-Infectious Cryptosporidium spp

Author(s) -

A. Alagappan,

Peter L. Bergquist,

Belinda C. Ferrari

Publication year - 2009

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00643-09

Subject(s) - cryptosporidium , cryptosporidium parvum , biology , fluorescence in situ hybridization , in situ , identification (biology) , fluorescence , microbiology and biotechnology , virology , computational biology , genetics , chemistry , ecology , feces , gene , optics , organic chemistry , chromosome , physics

A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification ofCryptosporidium parvum andC. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the majorCryptosporidium species of concern to public health.

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