Complete Sequence Analysis of Two Methanotroph-Specific repABC -Containing Plasmids from Methylocystis sp. Strain SC2 | Zendy

Bomba Dam | Zendy; Michael Kube | Zendy; Somasri Dam | Zendy; Richard Reinhardt | Zendy; Werner Liesack | Zendy

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Complete Sequence Analysis of Two Methanotroph-Specific repABC -Containing Plasmids from Methylocystis sp. Strain SC2

Author(s) -

Bomba Dam,

Michael Kube,

Somasri Dam,

Richard Reinhardt,

Werner Liesack

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00628-12

Subject(s) - plasmid , strain (injury) , methanotroph , sequence analysis , sequence (biology) , biology , microbiology and biotechnology , genetics , computational biology , gene , ecology , methane , anaerobic oxidation of methane , anatomy

The complete nucleotide sequences of two large, low-copy-number plasmids of 229.6 kb (pBSC2-1) and 143.5 kb (pBSC2-2) were determined during assembly of the whole-genome shotgun sequences of the methane-oxidizing bacteriumMethylocystis sp. strain SC2. The physical existence of the two plasmids in strain SC2 was confirmed by pulsed-field gel electrophoresis followed by Southern hybridization. Both plasmids have a conserved replication module of therepABC system and carry genes involved in their faithful maintenance and conjugation. In addition, they contain genes that might be involved in essential metabolic processes. These include several heavy metal resistance genes and copper transport genes in pBSC2-1 and a complete nitrous oxide reductase operon and apmoC singleton in pBSC2-2, the latter encoding the PmoC subunit of particulate methane monooxygenase.

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