Production of Resveratrol in Recombinant Microorganisms
Author(s) -
Jules Beekwilder,
Rianne Wolswinkel,
Harry Jonker,
Robert D. Hall,
C. H. Ric De Vos,
Arnaud Bovy
Publication year - 2006
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00609-06
Subject(s) - escherichia coli , resveratrol , yeast , saccharomyces cerevisiae , microorganism , recombinant dna , biochemistry , chemistry , dna ligase , biosynthesis , biology , microbiology and biotechnology , bacteria , enzyme , gene , genetics
Resveratrol production in Saccharomyces cerevisiae was compared to that in Escherichia coli. In both systems, 4-coumarate:coenzyme A ligase from tobacco and stilbene synthase from grapes were expressed. When p-coumaric acid was used as the precursor, resveratrol accumulations in the culture medium were observed to be comparable in E. coli (16 mg/liter) and yeast (6 mg/liter).
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