Development of an Efficient In Vivo System (P junc -TpaseIS  1223  ) for Random Transposon Mutagenesis of Lactobacillus casei | Zendy

Hélène Licandro | Zendy; Sophie Brinster | Zendy; Maarten van de Guchte | Zendy; Hélène Scornec | Zendy; Emmanuelle Maguin | Zendy; Philippe Sansonetti | Zendy; JeanFrançois Cavin | Zendy; Pascale Serror | Zendy

Open Access

Development of an Efficient In Vivo System (P _junc -TpaseIS ₁₂₂₃ ) for Random Transposon Mutagenesis of Lactobacillus casei

Author(s) -

Hélène Licandro,

Sophie Brinster,

Maarten van de Guchte,

Hélène Scornec,

Emmanuelle Maguin,

Philippe Sansonetti,

JeanFrançois Cavin,

Pascale Serror

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00531-12

Subject(s) - transposase , transposon mutagenesis , transposable element , lactobacillus casei , plasmid , electroporation , mutagenesis , biology , sleeping beauty transposon system , insertional mutagenesis , genetics , tn3 transposon , p element , mutant , dna , gene , bacteria

The random transposon mutagenesis system P(junc)-TpaseIS(1223) is composed of plasmids pVI129, expressing IS1223 transposase, and pVI110, a suicide transposon plasmid carrying the P(junc) sequence, the substrate of the IS1223 transposase. This system is particularly efficient in Lactobacillus casei, as more than 10,000 stable, random mutants were routinely obtained via electroporation.

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