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The random transposon mutagenesis system P(junc)-TpaseIS(1223) is composed of plasmids pVI129, expressing IS1223 transposase, and pVI110, a suicide transposon plasmid carrying the P(junc) sequence, the substrate of the IS1223 transposase. This system is particularly efficient in Lactobacillus casei, as more than 10,000 stable, random mutants were routinely obtained via electroporation.

applied and environmental microbiology

Development of an Efficient In Vivo System (P junc -TpaseIS 1223 ) for Random Transposon Mutagenesis of Lactobacillus casei