Cloning, Production, and Functional Expression of the Bacteriocin Enterocin A, Produced by Enterococcus faecium T136, by the Yeasts Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha, and Arxula adeninivorans | Zendy

Juan Borrero | Zendy; Gotthard Kunze | Zendy; Juan J. Jiménez | Zendy; Erik Böer | Zendy; Loreto Gútiez | Zendy; Carmen Herranz | Zendy; Luis M. Cintas | Zendy; Pablo E. Hernández | Zendy

Open Access

Cloning, Production, and Functional Expression of the Bacteriocin Enterocin A, Produced by Enterococcus faecium T136, by the Yeasts Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha, and Arxula adeninivorans

Author(s) -

Juan Borrero,

Gotthard Kunze,

Juan J. Jiménez,

Erik Böer,

Loreto Gútiez,

Carmen Herranz,

Luis M. Cintas,

Pablo E. Hernández

Publication year - 2012

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00530-12

Subject(s) - kluyveromyces lactis , pichia pastoris , enterococcus faecium , bacteriocin , biology , yeast , microbiology and biotechnology , kluyveromyces , cloning (programming) , pichia , aspergillus fumigatus , antimicrobial , biochemistry , saccharomyces cerevisiae , gene , recombinant dna , antibiotics , programming language , computer science

The bacteriocin enterocin A (EntA) produced by Enterococcus faecium T136 has been successfully cloned and produced by the yeasts Pichia pastoris X-33EA, Kluyveromyces lactis GG799EA, Hansenula polymorpha KL8-1EA, and Arxula adeninivorans G1212EA. Moreover, P. pastoris X-33EA and K. lactis GG799EA produced EntA in larger amounts and with higher antimicrobial and specific antimicrobial activities than the EntA produced by E. faecium T136.

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